Comparative genomic analysis, transcriptome and hydrocarbon-degrading capability of Vibrio sp. strain J502

Wells, Emma E.C. (2024) Comparative genomic analysis, transcriptome and hydrocarbon-degrading capability of Vibrio sp. strain J502. Masters thesis, Memorial University of Newfoundland.

	[English] PDF (Thesis) - Accepted Version Available under License - The author retains copyright ownership and moral rights in this thesis. Neither the thesis nor substantial extracts from it may be printed or otherwise reproduced without the author's permission. Download (4MB)
	[English] MS Word XML Document (docx) (File_S1_RStudio_code_GEA) - Accepted Version Available under License - The author retains copyright ownership and moral rights in this thesis. Neither the thesis nor substantial extracts from it may be printed or otherwise reproduced without the author's permission. Download (13kB)
	[English] MS Excel XML Document (xlsx) (Table_S1_Comparative_Phylogenetics) - Accepted Version Available under License - The author retains copyright ownership and moral rights in this thesis. Neither the thesis nor substantial extracts from it may be printed or otherwise reproduced without the author's permission. Download (17kB)
	[English] MS Excel XML Document (xlsx) (Table_S2_RNA_seq) - Accepted Version Available under License - The author retains copyright ownership and moral rights in this thesis. Neither the thesis nor substantial extracts from it may be printed or otherwise reproduced without the author's permission. Download (9kB)
	[English] MS Excel XML Document (xlsx) (Table_S3_Genes_Specific_Function) - Accepted Version Available under License - The author retains copyright ownership and moral rights in this thesis. Neither the thesis nor substantial extracts from it may be printed or otherwise reproduced without the author's permission. Download (930kB)
	[English] MS Excel XML Document (xlsx) (Table_S4_Transcriptomic_Summary V2) - Accepted Version Available under License - The author retains copyright ownership and moral rights in this thesis. Neither the thesis nor substantial extracts from it may be printed or otherwise reproduced without the author's permission. Download (807kB)
	[English] MS Excel XML Document (xlsx) (Table_S5_Genomic_Islands_Analysis) - Accepted Version Available under License - The author retains copyright ownership and moral rights in this thesis. Neither the thesis nor substantial extracts from it may be printed or otherwise reproduced without the author's permission. Download (58kB)

Abstract

Marine oil spills occurring as a result of anthropogenic activity pose a threat to marine birds, mammals, fish, and human health due to the toxicity of the compounds present in crude oil. Petroleum hydrocarbons are one of the most widespread organic contaminants impacting marine ecosystem, and contamination of marine sediments or coastal environments by hydrocarbons presents a major threat and challenge for remediation. Hydrocarbons in the marine environment are degraded abiotically by wave action, wind, and currents, and biotically by marine microorganisms. The bacterial genera Vibrio, Alteromonas, Bacillus and Pseudomonas are examples of species that have been identified as having hydrocarbon degrading metabolic capabilities. The genus Vibrio is extremely diverse and can be found in a wide variety of environments. Several environmental isolates of Vibrio spp. have been identified as hydrocarbon-degraders. Due to their widespread presence and adaptability, it is worthwhile studying the characteristics and metabolic traits of locally isolated Vibrio to understand their potential interaction with crude oil that is introduced into the marine environment. Here, I investigate the genomic, phylogenetic, and biochemical properties of a hydrocarbon-degrading marine bacterium isolated in Logy Bay, Newfoundland. Additionally, I determine the transcriptomic profile of this bacterium in crude oil exposure conditions and conduct an experiment to determine the chemical composition of chemically dispersed crude oil over time when it is incubated with a culture of this bacterium.

Actions (login required)

View Item