The histochemistry of the gastrointestinal tract of cod (Gadus morhua L.).

Yong, Neen-kee (1967) The histochemistry of the gastrointestinal tract of cod (Gadus morhua L.). Masters thesis, Memorial University of Newfoundland.

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This study was undertaken to correlate the histological features of the gastrointestinal tract of the cod with the occurrence of some of the hydrolytic enzymes and periodic acid-Schiff (PAS) reactive compounds. Representative tissue of nine regions of the tract (cardiac, fundic and pyloric stomach, pylorus, caeca, anterior loop of small intestine, posterior loop of small intestine, rectum and liver) were fixed in cold acetone, Lisen's fluid and Baker's formol calcium for the histology and the localization of alkaline phosphatase, acid phosphatase, esterase and PAS-positive substances. -- The distribution of alkaline phosphatase was mainly extracellular within the lamina propria and muscularis mucosae in the stomach region and in the striated border of the small intestine, caeca and rectum. The muscular layers, especially the outer longitudinal, indicated a very high activity of alkaline phosphatase. The acid phosphatase and esterase were intracellular and concentrated at the distal portion of the surface epithelium in the stomach and intestine. A high concentration of PAS-positive substances was observed in the gastric surface epithelial and intestinal goblet cells. It was not investigated whether or not a correlation exists between enzyme activity and age, sex of the animals or the season.

Item Type: Thesis (Masters)
Item ID: 9995
Additional Information: Bibliography : leaves 70-72.
Department(s): Science, Faculty of > Biology
Date: 1967
Date Type: Submission
Library of Congress Subject Heading: Codfish; Digestive organs--Fishes; Histochemistry.

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