Effects of lipid emulsions used in total parenteral nutrition and breast milk of lean and obese women, on adipogenesis in 3T3-L1 cells

Isesele, Peter. O. (2020) Effects of lipid emulsions used in total parenteral nutrition and breast milk of lean and obese women, on adipogenesis in 3T3-L1 cells. Masters thesis, Memorial University of Newfoundland.

[img] [English] PDF - Accepted Version
Available under License - The author retains copyright ownership and moral rights in this thesis. Neither the thesis nor substantial extracts from it may be printed or otherwise reproduced without the author's permission.

Download (2MB)


The growth of fat tissue in infants depends mainly on the differentiation and hypertrophy of existing adipocytes cells; thus, it is a sensitive period for the development of obesity. The objective of this thesis was to investigate the effects of lipid emulsions (Omegaven, SMOFlipid, and Intralipid) and breast milk (BM) on adipogenesis and lipolysis using 3T3-L1 cells. Intralipid, SMOFlipid, and Omegaven had omega (n)-6:n-3 polyunsaturated fatty acids (PUFA) of 7.8:1, 2.72:1 and 0.2:1, respectively. Upon treatment of 3T3-L1 cells with lipid emulsions, Omegaven treatment increased eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) levels in 3T3-L1 adipocytes, reduced triacylglycerol accumulation and inhibited the mRNA expression of lipogenic and lipolytic genes, compared to control or cells treated with SMOFlipid and Intralipid. BM from obese women (BMO) had higher n-6:n-3 PUFA compared to BM from lean women (BML). Upon treatment of 3T3-L1 cells, BML with high n-6:n-3 PUFA increased the mRNA expression of lipogenic genes; however, there was no effect of BMO lipogenesis. There was no effect of BML and BMO on the mRNA expression of lipolytic genes. Our findings suggest that exposure of preadipocytes to Omegaven prevents lipid accumulation and may be beneficial in the prevention of obesity. BMO had no effect suggesting other factors in BM may interfere with adipogenesis, and/or cause adipocyte dysfunction.

Item Type: Thesis (Masters)
URI: http://research.library.mun.ca/id/eprint/14419
Item ID: 14419
Additional Information: Includes bibliographical references. -- Restricted until 2022-04-01.
Keywords: Breast milk, Lipid emulsions, Adipogenesis, Lipogenesis, Polyunsaturated fatty acids, Infants, Obesity, Lipolysis
Department(s): Science, Faculty of > Biochemistry
Date: March 2020
Date Type: Submission
Digital Object Identifier (DOI): https://doi.org/10.48336/1B1M-K716
Library of Congress Subject Heading: Fat emulsions, Intravenous--Physiological effect; Breast milk--Physiological effect; Lipolysis.

Actions (login required)

View Item View Item


Downloads per month over the past year

View more statistics