Natural killer cells generate memory-type responses to human cytomegalovirus-infected fibroblasts

Newhook, Nicholas (2018) Natural killer cells generate memory-type responses to human cytomegalovirus-infected fibroblasts. Masters thesis, Memorial University of Newfoundland.

[img] [English] PDF - Accepted Version
Available under License - The author retains copyright ownership and moral rights in this thesis. Neither the thesis nor substantial extracts from it may be printed or otherwise reproduced without the author's permission.

Download (1MB)


Natural killer (NK) cells are cytotoxic lymphocytes that selectively respond against abnormal cells. Human cytomegalovirus (HCMV) infection causes expansion of NKG2C⁺CD57⁺ NK cells in vivo and NKG2C⁺ NK cells proliferate when cultured with HCMV-infected cells. This raises the possibility of an NK-cell subset selectively responding against a specific pathogen and accruing memory. To test this possibility, we compared proliferation, natural cytotoxicity and interferon-γ (IFN-γ) production of NK cells from HCMV-seropositive and HCMV-seronegative individuals co-cultured with HCMV-infected or uninfected MRC-5 cells. There was no significant difference in proliferation of NK cells from HCMV-seropositive or seronegative individuals against uninfected MRC-5 cells, but significantly more NK cells from the HCMV-seropositive groups proliferated in response to HCMV-infected MRC-5 cells. Natural cytotoxicity of NK cells against K562 cells increased following co-culture with HCMV-infected versus uninfected MRC-5 cells only for the HCMV-seropositive group. After co-culture with HCMV-infected MRC-5 cells, proliferating NK cells from HCMV-seropositive donors selectively produced IFN-γ when re-exposed to HCMV-infected MRC-5 cells. Both NKG2C⁺ and NKG2C⁻ NK cells proliferated in co-culture with HCMV-infected MRC-5 cells, with the fraction of proliferating NKG2C⁺ NK cells directly correlating with the circulating NKG2C⁺ fraction. These data illustrate an at least partly NKG2C-independent human NK-cell memory-type response against HCMV.

Item Type: Thesis (Masters)
Item ID: 13243
Additional Information: Includes bibliographical references (pages 63-71).
Keywords: Natural Killer Cells, NK cell memory, Cytomegalovirus, Immunology, NKG2C
Department(s): Medicine, Faculty of
Date: May 2018
Date Type: Submission
Library of Congress Subject Heading: Killer cells; Cytomegalovirus infections
Medical Subject Heading: Killer Cells, Natural; Cytomegalovirus Infections

Actions (login required)

View Item View Item


Downloads per month over the past year

View more statistics