Infection and propagation of hepatitis C virus in human CD4⁺ and CD8⁺ T lymphocytes in vitro

Skardasi, Georgia (2017) Infection and propagation of hepatitis C virus in human CD4⁺ and CD8⁺ T lymphocytes in vitro. Masters thesis, Memorial University of Newfoundland.

[img] [English] PDF - Accepted Version
Available under License - The author retains copyright ownership and moral rights in this thesis. Neither the thesis nor substantial extracts from it may be printed or otherwise reproduced without the author's permission.

Download (5MB)


Accumulated molecular and clinical evidence indicate that human immune cells can support replication of hepatitis C virus (HCV). The aim of this study was to investigate the ability of authentic, plasma-occurring HCV to infect human CD4⁺ and CD8⁺ T lymphocytes in vitro. For this purpose, we adopted the previously established in vitro HCV replication system in total T cells derived from cultured normal human PBMC by employing affinity-purified CD4⁺ and CD8⁺ T lymphocytes as targets. Using this system, we were able to demonstrate that molecularly intact HCV can infect and productively replicate in both CD4⁺ and CD8⁺ T lymphocytes, albeit at low levels, by documenting: (1) Presence of HCV positive and replicative (negative) strands in infected cells; (2) Intracytoplasmic localization of viral proteins; (3) Emergence of new HCV variants in the de novo infected cells, and (4) Release of HCV RNA-reactive particles from the infected cells with biophysical properties distinct from those of virions in inocula used to infect these cells.

Item Type: Thesis (Masters)
Item ID: 12638
Additional Information: Includes bibliographical references (pages 159-202).
Keywords: Hepatitis C, Lymphotropism, Lymphocytes, Immune cells, Occult Hepatitis C Infection
Department(s): Medicine, Faculty of
Date: May 2017
Date Type: Submission
Library of Congress Subject Heading: Hepatitis C virus; T cells
Medical Subject Heading: Hepacivirus; CD4-Positive T-Lymphocytes; CD8-Positive T-Lymphocytes; In Vitro Techniques

Actions (login required)

View Item View Item


Downloads per month over the past year

View more statistics