DNA methylation and gene expression in the runt and methyl-deprived pig

Collins, T. Mark (2016) DNA methylation and gene expression in the runt and methyl-deprived pig. Masters thesis, Memorial University of Newfoundland.

[img] [English] PDF - Accepted Version
Available under License - The author retains copyright ownership and moral rights in this thesis. Neither the thesis nor substantial extracts from it may be printed or otherwise reproduced without the author's permission.

Download (1MB)


This research focuses on studying the effect of reduced maternal methyl donor supply as well as intrauterine growth restriction (IUGR) on DNA methylation and on gene expression in pigs. Two separate techniques were used to determine global DNA methylation levels (cytosine extension and methyl incorporation assays) but no differences at the genomic level were identified for either experimental group using either technique. The expression levels of numerous genes involved in methylation directly or whose expression might be expected to change under these experimental conditions were also investigated. The expression of DNMT3a2 and BHMT were significantly lower in B6-deprived pigs but no differences were observed in folate-deprived or runt pigs compared to relevant controls. Further, sex differences were observed, indicating that sex should be considered for future studies regarding methyl-donor or runt-large studies in pigs. Lastly, we discovered an aberrant RNA species (ARS) in numerous pigs in both of our studies, from both Newfoundland and Manitoba research pig populations, and this ARS significantly affected the expression of numerous genes in both control and treatment groups.

Item Type: Thesis (Masters)
URI: http://research.library.mun.ca/id/eprint/12112
Item ID: 12112
Additional Information: Includes bibliographical references (pages 51-62).
Keywords: Epigenetics, Methylation, Pig, RT-qPCR
Department(s): Science, Faculty of > Biochemistry
Date: April 2016
Date Type: Submission
Library of Congress Subject Heading: DNA -- Methylation; Epigenetics; Fetal growth retardation -- Genetic aspects; Miniature pigs -- Diseases -- Genetic aspects

Actions (login required)

View Item View Item


Downloads per month over the past year

View more statistics