Regulation of Pygopus (hPygo2) expression in prostate cancer by E74-Like factor 1 (Elf-1) transcription factor

Gonzalez Aguirre, Paola (2016) Regulation of Pygopus (hPygo2) expression in prostate cancer by E74-Like factor 1 (Elf-1) transcription factor. Masters thesis, Memorial University of Newfoundland.

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Prostate cancer is a worldwide health concern. Pygopus2 (hPygo2) protein is required for growth in breast, ovarian, cervical and prostate cancer. hPygo2 expression is regulated by the Rb protein via the ETS factor Elf-1 in cervical and breast cancer. Additionally, the ETS family has confirmed roles in carcinogenesis and proliferation. The mechanism of hPygo2 expression has not been elucidated in prostate cancer. My hypothesis proposes that hPygo2 expression is regulated by Elf-1 bound to its promoter region. Prostate cancer cell lines were used to show protein levels of hPygo2, Elf-1 and ETS. ChIP assays confirmed varying binding capability of Elf-1 and ETS factors to the proximal promoter region between cell lines. Elf-1 knockdown experiments were performed, results show no change in hPygo2 protein levels but show reduction in 22Rv1 mRNA levels. These results suggest that Elf-1 might not be exclusively involved in the activation of Pygopus expression in prostate cancer.

Item Type: Thesis (Masters)
Item ID: 11945
Additional Information: Includes bibliographical references (pages 57-71).
Keywords: Pygopus, Prostate Cancer, Cancer, ETS factors, Elf-1, hPygo2, Prostate
Department(s): Medicine, Faculty of > Biomedical Sciences
Date: May 2016
Date Type: Submission
Library of Congress Subject Heading: Prostate--Cancer--Genetic aspects; Cellular signal transduction; Transcription factors; Cancer cells--Growth--Regulation
Medical Subject Heading: Prostate Neoplasms--genetics; Signal Transduction; Transcription Factors; Intracellular Signaling Peptides and Proteins

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