Studies on the proteins of the plasma portion of the blood of the Ommastrephid squid, Illex Illecebrosus Illecebrosus (Leseur, 1821)

Barnes, David Hugh (1968) Studies on the proteins of the plasma portion of the blood of the Ommastrephid squid, Illex Illecebrosus Illecebrosus (Leseur, 1821). Masters thesis, Memorial University of Newfoundland.

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Abstract

Specimens of the Ommastrephid squid Illex illecebrosus illecebrosus LeSueur were captured at two locations on the Avalon Peninsula of the island of Newfoundland, from July to October, 1966. A blood sample was obtained from each of the more than 400 specimens immediately upon capture. The blood samples were each centrifuged in the laboratory to remove cellular materials, and the plasma samples thereby obtained were stored at -20°C. Samples of this plasma were examined refractometrically for total protein content and subjected to electrophoretic analyses on a cellulose acetate medium. Antiserum to squid plasma was raised in a rabbit, and immuno-diffusion tests were carried out, also on cellulose acetate. The total protein values obtained ranged from 11.30 to 14.50 g/100 ml of plasma, the average being 12.76 g/100 ml. The electrophoretic analyses revealed two closely associated protein components, at least one of which is believed to be the proteinaceous part of the respiratory pigment, hemocyanin. The immuno-diffusion tests indicated the presence in the blood plasma of the squid of at least one antigenic component, probably hemocyanin.

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