Diversity analysis of blueberry clones and cultivars using EST-PCR and microsatellite markers

Tailor, Sapan Pravinbhai (2016) Diversity analysis of blueberry clones and cultivars using EST-PCR and microsatellite markers. Masters thesis, Memorial University of Newfoundland.

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Abstract

The blueberry (Vaccinium spp. L.) is a health promoting and economically important fruit crop. Genetic diversity and relatedness were studied in 63 blueberry genotypes, using eleven EST–PCR and nine microsatellite markers. Markers found to be polymorphic and detected 436 and 272 alleles, respectively. Other parameters such as PIC, expected and observed heterozygosity were also high for both the markers. Cluster analyses such UPGMA, NJ and PCO identified clustering of genotypes according to their geographic collection site. Fingerprinting data was used for the STRUCTURE analysis which also separated clones and cultivars based on their geographic collection site. Similarity was found among the results of UPGMA, NJ, PCoA and STRUCURE analyses for both the markers analysed individually and combined. AMOVA analysis revealed high genetic diversity among genotypes. Results from the present study will help in germplasm management and for evaluating and selecting promising blueberry genotypes in the current blueberry improvement program.

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