Cloning and characterization of a cDNA encoding er1, a novel developmentally regulated FGF response gene

Li, Yu (1998) Cloning and characterization of a cDNA encoding er1, a novel developmentally regulated FGF response gene. Masters thesis, Memorial University of Newfoundland.

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    Available under License - The author retains copyright ownership and moral rights in this thesis. Neither the thesis nor substantial extracts from it may be printed or otherwise reproduced without the author's permission.
    (Original Version)

Abstract

In order to investigate the molecular mechanism of mesoderm induction by FGF in Xenopus Laevis, I have utilized the polymerase chain reaction (PCR)-based differential display methodology (Liang and Pardee, 1992) to identity a novel transcript whose expression level increased in Xenopus embryo explants during mesoderm induction by fibroblast growth factor (FGF). The PCR product was used to clone a 2.3-kb cDNA representing this transcript, which I have named er1. The er1 cDNA contains a single open reading frame (ORF) predicted to encode a protein of 493 amino acid residues. Northern blot analysis revealed a single 2.8-kb mRNA that was observed predominantly during the initial cleavage and blastula stages of Xenopus development, with little or no detected mRNA during subsequent development. In vitro translation of er1 using a rabbit reticulocyte lysate system produced a protein with an apparent molecular mass of 74kDa. A database homology search revealed that the predicted er1 amino acid sequence contains three regions of similarity to the rat metastasis-associated gene mta1. FGF is known to play an important role in both mesoderm induction and gastrulation movement during amphibian development, elucidation of the function of this mta1-related FGF response gene may lead to a better understanding of the early development of Xenopus Laevis.

Item Type: Thesis (Masters)
URI: http://research.library.mun.ca/id/eprint/852
Item ID: 852
Additional Information: Bibliography: leaves 85-97
Department(s): Medicine, Faculty of
Date: 1998
Date Type: Submission
Library of Congress Subject Heading: Fibroblast growth factors; Xenopus laevis; Molecular cloning; Fibroblast Growth Factors; Xenopus laevis; Cloning, Molecular

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