HLA Class II expression on breast cancer cells

Edgecombe, Allison D. (2002) HLA Class II expression on breast cancer cells. Masters thesis, Memorial University of Newfoundland.

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Abstract

Presentation of tumor peptides by HLA molecules is required for immune system recognition and eradication of tumors. This study characterized 11 breast cancer cell lines (BCCLs) as potential antigen presenting cells, assessed expression of CIITA, a regulator of HLA class II and co-chaperone transcription, and examined expression of HLA-DR allelic products in these cells. -- Gene transcription was determined by RT-PCR. Protein expression was demonstrated using monoclonal antibodies and flow cytometry and immunocytochemistry. -- Unexpectedly, constitutive HLA class II and/or co-chaperone protein expression was detected in 5 of 11 BCCLs. Following IFN-γ treatment, most cell lines up-regulated HLA class II and co-chaperones and the co-stimulatory molecule CD40 suggesting a capacity for HLA class II mediated antigen presentation. -- Several BCCLs constitutively expressed HLA class II and co-chaperones in the absence of CIITA suggesting CIITA independent regulation of these genes in BCCLs. -- Some cell lines selectively up-regulated certain HLA-DR allelic products. Since all HLA-DR alleles were transcribed following IFN-γ treatment, post-transcriptional events may be implicated in this selective expression. -- Failure to express HLA class II antigens and co-stimulatory molecules may allow tumors to escape immune recognition.

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