Short term and long term effects of curcumin on activities of glutathione S-transferase and cytochrome P450 in livers of rats

Husain, Saleha (1997) Short term and long term effects of curcumin on activities of glutathione S-transferase and cytochrome P450 in livers of rats. Masters thesis, Memorial University of Newfoundland.

[English] PDF - Accepted Version Available under License - The author retains copyright ownership and moral rights in this thesis. Neither the thesis nor substantial extracts from it may be printed or otherwise reproduced without the author's permission. Download (4MB)

Abstract

Curcumin, a yellow compound naturally occurring in the plant Curcuma longa Linn, was studied for its in vivo short term and long term effects on the activities of some glutathione S-transferase (GST) and cytochrome P450 isozymes in livers of rats. The rats were fed 1% curcumin in an AIN 76 diet which contained 15% protein for a duration of approximately two weeks for short term studies and for a duration of approximately six weeks for long term studies. Glutathione S-transferase (GST) activities were measured in liver cytosols by using 1-chloro-2,4-dinitrobenzene (CDNB) and 1,2-dichloro-4-nitrobenzene (DCNB) as substrates. Cytochrome P450 1A1/2 and 2B1 activities were measured as ethoxyresorufin deethylation (EROD) and pentoxyresorufin depentylation (PROD) respectively in liver microsomes. Curcumin treatment was found to significantly inhibit GST activities in the long term studies as compared to controls when CDNB and DCNB were used as substrates. There was no significant inhibition of glutathione S-transferase (GST) activities by curcumin in the short term studies, as compared to controls. Curcumin treatment in both the short term and long term studies showed no significant effects on cytochrome P450 IAI/2 and 2Bl activities, as compared to controls. In vitro, curcumin was found to be a potent inhibitor of cytochrome P450 1A1/2 and 2B1 activities. These results suggest that short term administration of curcumin can lead to no significant effects on glutathione S-transferase (GST) and cytochrome P450 activities. However, long term administration of curcumin can lead to inhibition of glutathione S-transferase activities (GST) but no significant effects on cytochrome P450 activities.

Actions (login required)

View Item