Extraction techniques and the chromatographic determination of lipids in cold water marine species

Hooper, Tara Suzanne (2006) Extraction techniques and the chromatographic determination of lipids in cold water marine species. Masters thesis, Memorial University of Newfoundland.

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Abstract

The exhaustive extraction and detection of lipids from marine plant and animal tissues is challenging due to the chemical variability among different lipid classes. An automated procedure based on the Randall method, in which the sample is directly immersed in boiling solvent followed by a solvent wash, has been developed for the exhaustive extraction of lipids from macrophytes and fish. The extraction of wet tissues is obtained by using a solvent system composed of chloroform, methanol and water. The efficiency of this procedure is comparable to traditional extraction methods and it is especially useful for large sample loads. -- For the determination of lipids in very small samples such as individual fish larva, short column GC/FID provides an excellent alternative to the more commonly used Iatroscan TLC/FID, due to its lower detection limit as well as its ability to profile lipids based on their carbon number. Results reveal that there were no significant differences in the quantification of triacylglycerols or sterols in individual fish larvae (p > 0.05); however, GC/FID is more sensitive, precise, rapid and cost-efficient. Further, with the use of a guard column, the short column GC/FID method has been further expanded to include the qualitative analysis of macrophytes.

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