Zhao, Cida (1994) Effects of adrenergic drugs and monosodium glutamate on the development of sex-dependent cytochrome P450 system in male rat liver. Masters thesis, Memorial University of Newfoundland.
PDF (Migrated (PDF/A Conversion) from original format: (application/pdf))
- Accepted Version
Available under License - The author retains copyright ownership and moral rights in this thesis. Neither the thesis nor substantial extracts from it may be printed or otherwise reproduced without the author's permission.
The effects of adrenergic drugs on the development of male-specific cytochrome P450 in the male rat liver were studied. Pups, castrated on Day 0, were given (icv) α-antagonist or β-agonist with or without testosterone propionate (sc.), and assayed on Day 65. Other pups were administered with (icv) adrenergic agonists or antagonists on Day 0 & 3 of life, castrated on Day 55, and assayed on Day 70. Neither α-antagonist nor β-agonist defeminized the EMDM activity in neonatally castrated rats. In the rats castrated on Day 55, β-antagonist and α₁-antagonist superdefeminized the EMDM activity. β-antagonist increased the total amount of P450 but α₁-antagonist did not. In addition, the Vmax of EMDM in β-antagonist-treated animals was increased; the Km was unchanged. Further, β-antagonist stimulated the activities of 16α-, 2α-, and 6β-testosterone hydroxylases in the superdefeminized rats. However, both β-antagonist and α₁-antagonist, given simultaneously, suppressed the superdefeminization of EMDM activity. -- The effect of monosodium glutamate (MSG) on the prepubertal development of male-specific cytochrome P450 in rat liver were also studied. Pups were treated on Day 1-9 with 2 or 4 mg/g MSG (sc), and on Day 35 the EMDM activities in 2 and 4 mg/g MSG-treated groups were the same as control values. However, MSG stimulated, dose-dependently, the EMDM activity on Day 65. In addition, Vmax of EMDM was elevated in MSG-treated group; the Km was unchanged. Finally, 4 mg/g MSG stimulated the activity of 6β- but not 16α/2α-testosterone hydroxylases.
|Item Type:||Thesis (Masters)|
|Additional Information:||Bibliography: leaves 115-131.|
|Department(s):||Pharmacy, School of|
|Library of Congress Subject Heading:||Cytochrome P-450; Monosodium glutimate--Physiological effect; Adrenaline--Receptors--Physiological effect; Liver--Effect of drugs on|
|Medical Subject Heading:||Cytochrome P-450 Enzyme System; Sodium Glutamate; Receptors, Adrenergic--physiology; Liver--drug effects|
Actions (login required)