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As a major component of the innate immune system, natural killer cells are responsible for activating the cytolytic killing of certain pathogen-infected or tumor cells. The self-recognition of natural killer cells is achieved in part by the killer cell immunoglobulin-like receptors (KIRs) protein family. In the current study, using a suite of biophysical methods, we investigate the self-association of an activating KIR, KIR2DS1. This KIR is of particular interest because when in the presence of the HLA-Cw6 protein, KIR2DS1 becomes a major risk factor for psoriasis, an autoimmune chronic skin disease. Using circular dichroism spectroscopy, dynamic light scattering, and atomic force microscopy, we reveal that KIR2DS1 self-associates in a well-defined fashion. Our novel results on an activating KIR allow us to suggest a working model for the KIR2DS1- HLA class I molecular mechanism.
|Keywords:||HLA antigen class 1, Natural killer cell receptor, Natural killer cell receptor KIR2DS1, Unclassified drug, Article, Innate immunity, Molecular mechanics, Oligomerization, Protein analysis, Protein assembly, Protein conformation, Protein expression, Protein purification, Protein structure|
|Department(s):||Science, Faculty of > Biochemistry
Science, Faculty of > Physics and Physical Oceanography
|Date:||30 August 2011|
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