Cold adaptation of Atlantic salmon tropomyosin: role of residue '77'

Ige, Tolulope Oluwasomo (2012) Cold adaptation of Atlantic salmon tropomyosin: role of residue '77'. Masters thesis, Memorial University of Newfoundland.

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Abstract

Tropomyosin from the fast skeletal (trunk) muscle of Atlantic salmon (Salmo salar) is an alpha-type isoform. It shares ~93% identity with a counterpart from rabbit skeletal muscle (20 amino acid substitutions out of a total of 284) but is less thermally stable. -- An interesting aspect of the small heterogeneity is the replacement of lysine-77 in rabbit with threonine in salmon. In the case of the mammalian protein, the lysine in question is positioned to form ion pairs with aspartate-80 in the next turn of the same helix and also with glutamate 82 in the opposing helix of the coiled coil. Thus, at neutral pH, salmon tropomyosin loses two potential charge-charge (ion pair) interactions. The contribution of residue-77 to the properties of salmon tropomyosin has been investigated by mutating it to the amino acid in rabbit using site directed mutagenesis, followed by circular dichroism, differential scanning calorimetry, affinity chromatography and limited proteolysis. A major finding of this research is that threonine in the 77th position of salmon tropomyosin is destabilizing compared to lysine in the same position. Reducing the number of ion pairs is concluded to be part of the structural means by which tropomyosin, and possibly other rod-shaped proteins, adapt to low temperature. The specific details are outlined below in point form: -- 1. Atlantic salmon fast skeletal muscle alpha-tropomyosin cDNA was mutated to replace the threonine at position 77 with lysine using the QuickChange Lightning site directed mutagenesis kit. The mutation was confirmed by DNA sequencing. -- 2. Mutated and non mutated recombinant tropomyosins were obtained by expression in E. coli BL21 cells and chromatographically isolated. -- 3. The mutant salmon tropomyosin, containing Lys-77, exhibited a faster electrophoretic mobility than the non-mutant (Thr-77), in the presence of the detergent sodium dodecyl sulfate, which is attributable to a difference in detergent binding due to the extra charge. This electrophoretic shift was useful in the identification of amino-terminal peptides (see below, #7). -- 4. Far-UV circular dichroism was used to investigate the conformational stability of the recombinant salmon tropomyosins (ionic strength 0.1 M, +DTT, pH 7.0). The observed melting temperatures of the mutant (~45°C) and non mutant (~40°C) recombinant proteins differ by approximately 5°C. -- 5. Differential scanning calorimetry was employed to monitor the thermal unfolding of non mutant and mutant recombinant tropomyosin (ionic strength 0.1 M, +DTT, pH 7.0) by direct heating. The observed melting temperatures of the mutant (~44°C) and non mutant (~40.5°C) protein differ by approximately 4°C. -- 6. In an affinity chromatography experiment mutant tropomyosin bound more strongly to troponin-Sepharose 4B than the non mutant. -- 7. The time-course of limited chymotryptic or tryptic digestion (T, 25°C) was monitored by SDS PAGE and found to be similar for mutant and non-mutant tropomyosins. Edman-based sequencing showed that the initial chymotryptic cleavage site in salmon recombinant tropomyosin (both mutant and non-mutant) is between Leu-11 and Lys-12. The second chymotryptic site is between Leu-169 and Val-170 which corresponds to the preferred site reported previously in rabbit alpha-tropomyosin.

Item Type: Thesis (Masters)
URI: http://research.library.mun.ca/id/eprint/10716
Item ID: 10716
Additional Information: Includes bibliographical references (leaves 78-96).
Department(s): Science, Faculty of > Biochemistry
Date: 2012
Date Type: Submission
Library of Congress Subject Heading: Atlantic salmon--Effect of cold on; Tropomyosins; Lysine; Animal mutation.

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