Tandem mass spectrometric analysis of bacterial lipid A of Aeromonas Salmonicida (SJ-112)

Alarabi, Faisal (2015) Tandem mass spectrometric analysis of bacterial lipid A of Aeromonas Salmonicida (SJ-112). Masters thesis, Memorial University of Newfoundland.

[img] [English] PDF - Accepted Version
Available under License - The author retains copyright ownership and moral rights in this thesis. Neither the thesis nor substantial extracts from it may be printed or otherwise reproduced without the author's permission.

Download (5Mb)

Abstract

This study will present an interpretation of the mass spectrometry gas-phase fragmentation patterns of the extracted Lipid A that is obtained from the native LPS extracts isolated from the marine Gram-negative bacteria Aeromonas Salmonicida (SJ-112). It is known that the surface antigen lipopolysaccarides (LPS) SJ-112 infect various fish species (Atlantic salmon and cod) which are cultivated in aquaculture ventures. The exact molecular structure of the Lipid A has not yet been precisely established. This thesis will present the mass spectrometric fingerprint identification and structural elucidation of the Lipid A from A. Salmonicida, which are carried out by using mass spectrometry techniques namely, electrospray ionization tandem mass spectrometry (ESI-MS/MS) using an FT-ICR instrument and matrix assisted laser desorption ionization tandem mass spectrometry (MALDI-MS/MS) using a TOF/TOF instrument. The concomitant uses of high-energy (CID-MS/MS) and low-energy collision induced dissociation (CID-MS/MS) analysis were also used to elucidate the MS/MS fingerprints of this complex biomolecule and can be effectively used for any quantitative or qualitative studies.

Item Type: Thesis (Masters)
URI: http://research.library.mun.ca/id/eprint/8511
Item ID: 8511
Additional Information: Includes bibliographical references (pages 130-151).
Keywords: Tandem Mass Spectrometric, Bacterial Lipid A, Gram negative bacteria, matrix assisted laser desorption ionization tandem mass spectrometry (MALDI-MS/, matrix assisted laser desorption ionization tandem mass spectrometry (MALDI-MS/M
Department(s): Science, Faculty of > Chemistry
Date: June 2015
Date Type: Submission
Library of Congress Subject Heading: Microbial lipids--Analysis; Aeromonas salmonicida; Tandem mass spectrometry; Electrospray ionization mass spectrometry

Actions (login required)

View Item View Item

Downloads

Downloads per month over the past year

View more statistics