Tandem mass spectrometric analysis of bacterial lipid A and synthetic glycosyl donors containing the 2-amino-2-deoxy-D-glucosaminyl moiety

Allehyane, Bashaeer (2014) Tandem mass spectrometric analysis of bacterial lipid A and synthetic glycosyl donors containing the 2-amino-2-deoxy-D-glucosaminyl moiety. Masters thesis, Memorial University of Newfoundland.

[img] [English] PDF - Accepted Version
Available under License - The author retains copyright ownership and moral rights in this thesis. Neither the thesis nor substantial extracts from it may be printed or otherwise reproduced without the author's permission.

Download (2941Kb)

Abstract

This study presents an interpretation of the mass spectrometry gas-phase fragmentation patterns of the extracted lipid A obtained from the native LPS extracts isolated from Aeromonas Liquefaciencs. This LPS SJ-19 is known to infect various fish species (Atlantic salmon and cod) which are cultivated in aquaculture ventures. The exact molecular structure of the lipid A and the core oligosaccharide region of this Rough bacterium have not yet been precisely established. This thesis presents the mass spectrometric fingerprint identification and structural elucidation of the lipid A from A. Liquefaciencs, which was accomplished via the use of electrospray ionization tandem mass spectrometry (ESI-MS/MS using FTICR, QIT, QHQ-MS/MS) and matrix assisted laser desorption ionization tandem mass spectrometry (MALDI-MS/MS). In this work, tandem-in-time and tandem-in-space mass spectrometry was chosen for the structural elucidation of the lipid A isolated from A. Liquefaciencs SJ-19. The concomitant uses of high-energy and low-energy collision induced dissociation (CIDMS/ MS) analysis were also used to elucidate the MS/MS fingerprints of this complex biomolecule and can be effectively used for any quantitative or qualitative studies. In addition, a novel MS/MS study of a series of 2-acylated-2-deoxy-Dgluosamine glycosyl donors is also presented using ESI-MS/MS tandem-in-space and tandem-in-time mass spectrometry.

Item Type: Thesis (Masters)
URI: http://research.library.mun.ca/id/eprint/8334
Item ID: 8334
Additional Information: Includes bibliographical references (pages 172-182).
Keywords: MALDI, FTICR, ESI, MS, MSMS
Department(s): Science, Faculty of > Chemistry
Date: 15 September 2014
Date Type: Submission
Library of Congress Subject Heading: Endotoxins--Analysis; Tandem mass spectrometry; Endotoxins--Structure; Oligosaccharides--Synthesis

Actions (login required)

View Item View Item

Downloads

Downloads per month over the past year

View more statistics