Chavoshi Jolfaei, Mahin S. (2014) Evaluation of foxO modulation in modelling Parkinson Disease in Drosophila melanogaster. Masters thesis, Memorial University of Newfoundland.
- Accepted Version
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Symptoms of Parkinson Disease (PD), the second most common neurodegenerative disease, emerge due to degeneration of dopaminergic neurons. Approximately 10% of PD is familial with a number of genes that have been recognized to play a role. In 2012, a genome wide study revealed a role for the foxO transcription factor in PD. To more fully understand human diseases, model organisms such as Drosophila melanogaster are widely used. In the present study, I have attempted to model Parkinson Disease in Drosophila by foxO modulation using RNAi transgenes. To achieve this goal, I conducted longevity assays and locomotion measurements along with supportive experiments that target expression in the developing eye. Results suggest that under certain conditions, slight elevation of foxO by down-regulation of one of foxO’s inhibitors, the kinase minibrain (mnb), can model PD in flies. Results are presented here showing that expression of mnb-RNAi (and predicted subsequent slight elevation of foxO) in dopaminergic neurons results in significant loss of climbing ability: the defining feature of PD models in fruit flies. Other results suggest that slight decrease of foxO by foxO-RNAi decreases life span significantly when expressed under the control of TH-Gal4( Tyrosine Hydroxylase-Gal4) . In addition, results show that GFP-RNAi expression under the control of TH-Gal4 reduces life span significantly.
|Item Type:||Thesis (Masters)|
|Additional Information:||Includes bibliographical references (pages 54-58).|
|Department(s):||Science, Faculty of > Biology|
|Library of Congress Subject Heading:||Drosophila melanogaster--Diseases--Genetic aspects; Parkinson's disease--Animal models; Forkhead transcription factors|
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