Steroid metabolism in essential hypertension

Dey, Arun Chandra (1974) Steroid metabolism in essential hypertension. Masters thesis, Memorial University of Newfoundland.

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    Available under License - The author retains copyright ownership and moral rights in this thesis. Neither the thesis nor substantial extracts from it may be printed or otherwise reproduced without the author's permission.
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Abstract

Alterations of steroid metabolism in hypertension have been investigated with male and female normal and essential hypertensive subjects. Three groups of urinary metabolites have been quantitated: 1. androsterone, etiocholanolone and dehydroepiandrosterone (11-deoxy-17-ketosteroids, C₁₉O₂-KS), 2. 11-keto and 11β-hydroxy androsterone and 11-keto and 11β-hydroxy etiocholanolone (11-oxy-17-ketosteroids, C₁₉O₃-KS), and 3. 17-ketogenic steroids (KGS) oxidised to etiocholanolone, 11β-hydroxy androsterone and 11β-hydroxy etiocholanolone. The urinary steroids were hydrolysed enzymatically, fractionated on silica gel columns and estimated as trimethyl silyl ether derivatives by gas liquid chromatography. -- The following statistically significant differences were found in the excretion of metabolites by normal and hypertensive subjects. -- 1. C₁₉O₂-17KS fraction: the excretion of etiocholanolone glucuronide and dehydroepiandrosterone sulfate was markedly reduced in the hypertensive group (P 0.001), and the ratio of the 5α/5β-reduced metabolites was higher (P 0.001). The latter suggested a relative deficiency of 5β-reductase activity in the hypertensive group. -- 2. C₁₉O₃-17KS fraction: the excretion of the 5α-reduced metabolites was higher (P 0.01) in the hypertensive group, and the 5α/5β ratio was also increased (P 0.01), due to the increased 5α-compounds. No significant reduction was found for the 5β-reduced metabolites. -- 3. KGS fraction: the excretion of both 5α- and 5β- reduced compounds was higher (P 0.05) in the hypertensive group, but the 5β-compounds predominated in both normal and hypertensive groups. An increased excretion of 5β-reduced metabolites may suggest that the 5β-reductase activity is more active in the metabolism of the 17-ketogenic steroids than C₁₉O₂-17KS. -- The influence of the "11-oxy" function and corticosteroid "Side chain" on the relative 5α- and 5β- reductase enzyme activities and the influence of blood pressure has been discussed. -- Comparison of the proportions of reduced metabolites in the urine of normal and essential hypertensive subjects indicates that they are relatively unaffected by age, sex or intensity of blood pressure.

Item Type: Thesis (Masters)
URI: http://research.library.mun.ca/id/eprint/7369
Item ID: 7369
Additional Information: Bibliography: leaves [99]-138.
Department(s): Medicine, Faculty of
Date: 1974
Date Type: Submission
Library of Congress Subject Heading: Hypertension; Steroids--Metabolism
Medical Subject Heading: Hypertension--metabolism; Steroids--metabolism

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