The impact of ozonation of Atlantic cod, Atlantic salmon and rainbow trout eggs on hatching success, larval growth and survival

Fry, Jessica S. (2014) The impact of ozonation of Atlantic cod, Atlantic salmon and rainbow trout eggs on hatching success, larval growth and survival. Masters thesis, Memorial University of Newfoundland.

[img] [English] PDF (Migrated (PDF/A Conversion) from original format: (application/pdf)) - Accepted Version
Available under License - The author retains copyright ownership and moral rights in this thesis. Neither the thesis nor substantial extracts from it may be printed or otherwise reproduced without the author's permission.

Download (3319Kb)
  • [img] [English] PDF - Accepted Version
    Available under License - The author retains copyright ownership and moral rights in this thesis. Neither the thesis nor substantial extracts from it may be printed or otherwise reproduced without the author's permission.
    (Original Version)

Abstract

The direct exposure of eggs to ozonated water has generated interest as a means of ensuring pathogen-free eggs without the use of harsh chemicals. However, there are numerous knowledge gaps including safe contact times, exposure levels in both fresh water and sea water, and as potential long-term effects on aquaculture species such as Atlantic cod, Atlantic salmon, and rainbow trout. The effect of different ozone doses (0.5- 1.0, 1.5- 2.0 and 2.5- 3.0 mg O3 L⁻¹ for 1.5 min) was tested on recently fertilized Atlantic cod and eyed salmonid eggs vis-à-vis the commercial disinfectant Perosan™ (0.004 mg L⁻¹) and Ovadine (100 mg L⁻¹) as well as an untreated control (similar handling and husbandry). The impact of ozone application was evaluated based on larval nucleic acid concentration, hatching success, larval growth, and survival. Overall, the study showed no negative effect of ozonation at < 3.0 mg O3 L⁻¹ for 1.5 min on cod eggs, cod larvae (up to 30 dph), and Atlantic salmon and rainbow trout eggs monitored until 85% yolk sac depletion. The ability of ozone to eradicate Saprolegnia diclina infection on salmonid eggs as well the impact of ozone disinfection on S. diclina in the absence of salmonid eggs were also investigated. Results suggest that ozonation of S. diclina hyphal mats can reduce the growth of the fungus, with the level of impact being dependent on volume of S. diclina initially present.

Item Type: Thesis (Masters)
URI: http://research.library.mun.ca/id/eprint/6502
Item ID: 6502
Additional Information: Includes bibliographical references (pages 79-89).
Department(s): Science, Faculty of > Aquaculture
Date: May 2014
Date Type: Submission
Library of Congress Subject Heading: Ozonization; Atlantic cod--Eggs--Incubation; Atlantic salmon--Eggs--Incubation; Rainbow trout--Eggs--Incubation; Atlantic cod--Larvae--Growth; Atlantic salmon--Larvae--Growth; Rainbow trout--Larvae--Growth; Atlantic cod--Larvae--Mortality; Atlantic salmon--Larvae--Mortality; Rainbow trout--Larvae--Mortality

Actions (login required)

View Item View Item

Downloads

Downloads per month over the past year

View more statistics