Strong, Vanessa Dawn (2014) Molecular correlates of multiday memory in an appetitive conditioning model: insights into mediators of memory extension. Masters thesis, Memorial University of Newfoundland.
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These experiments provide insight into potential mediators implicated in the maintenance of memory duration using an olfactory preference learning paradigm. Neonatal rats were trained in a single training session by pairing odor (CS) with pharmacological agents (UCS) to promote odor preference of different durations (24h, 4-Day and 5-Day). For the first time in olfactory preference learning we characterize the pCREB expression profile as biphasic with peaks at 10min and 2h post-conditioning in 24h and 5-Day olfactory preference memory. Second, we show histone acetylation is enhanced by HDAC inhibition via NaB in both learning and non-learning conditions. Finally, q-PCR reveals CREB target genes Nr4a1 and Egr-1 are differentially expressed across 24h and 5-Day models, consistent with a possible role in memory extension. These experiments support CREB as a possible initiator in mediating downstream events leading to the changes in synaptic plasticity that accompany extension of long-term memory.
|Item Type:||Thesis (Masters)|
|Additional Information:||Includes bibliographical references (pages 77-90).|
|Department(s):||Medicine, Faculty of|
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