Novel regulatory roles of omega-3 fatty acids in metabolic pathways: a proteomics approach

Ahmed, Abeer A. and Balogun, Kayode A. and Bykova, Natalia V. and Cheema, Sukhinder K. (2014) Novel regulatory roles of omega-3 fatty acids in metabolic pathways: a proteomics approach. Nutrition & Metabolism, 11 (6). ISSN 1743-7075

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Abstract

Background: Omega-3 polyunsaturated fatty acids (n-3 PUFA) have been shown to alleviate the symptoms of metabolic disorders, such as heart disease, diabetes, obesity and insulin resistance. Several putative mechanisms by which n-3 PUFA elicit beneficial health effects have been proposed; however, there is still a shortage of knowledge on the proteins and pathways that are regulated by n-3 PUFA. Methods: Using two dimensional polyacrylamide gel electrophoresis (2D-PAGE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis, we investigated the effects of diets high or low in n-3 PUFA on hepatic proteomic profile of C57BL/6 mice. Results: The findings show for the first time that high dietary n-3 PUFA reduced the expression of regucalcin, adenosine kinase and aldehyde dehydrogenase. On the other hand, diets high in n-3 PUFA increased the expression of apolipoprotein A-I, S-adenosylmethionine synthase, fructose-1, 6-bisphosphatase, ketohexokinase, malate dehydrogenase, GTP-specific succinyl CoA synthase, ornithine aminotransferase and protein disulfide isomerase-A3. Conclusions: Our findings revealed for the first time that n-3 PUFA causes alterations in several novel functional proteins involved in regulating lipid, carbohydrate, one-carbon, citric acid cycle and protein metabolism, suggesting integrated regulation of metabolic pathways. These novel proteins are potential targets to develop therapeutic strategies against metabolic disorders.

Item Type: Article
URI: http://research.library.mun.ca/id/eprint/6435
Item ID: 6435
Additional Information: Memorial University Open Access Author's Fund
Department(s): Science, Faculty of > Biochemistry
Date: 17 January 2014
Date Type: Publication
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