Poduska, Kristin M. and Kumar, M. Ramesh and Merschrod S., Erika F. (2009) Correlating mechanical properties with aggregation processes in electrochemically fabricated collagen membranes. Biomacromolecules , 10 (7). pp. 1970-1975. ISSN 1526-4602
PDF (Migrated (PDF/A Conversion) from original format: (application/pdf))
- Accepted Version
Available under License Creative Commons Attribution Non-commercial.
We show that mechanical stiffness is a useful metric for characterizing complex collagen assemblies, providing insight about aggregation products and pathways in collagen-based materials. This study focuses on mechanically robust collagenous membranes produced by an electrochemical synthesis process. Changing the duration of the applied electric field, or adjusting the electrolyte composition (by adding Ca2+, K+, or Na+ or by changing pH), produces membranes with a range of Young’s moduli as determined from force-displacement measurements with an atomic force microscope. The structural organization, characterized by UV−visible spectroscopy, Raman spectroscopy, optical microscopy, and atomic force microscopy, correlates with the mechanical stiffness. These data provide insights into the relative importance of different aggregation pathways enabled by our multiparameter electrochemically induced collagen assembly process.
|Keywords:||Young’s modulus, force curves, protein aggregation, fibrillogenesis, collagen, electrosynthesis, Raman spectroscopy|
|Department(s):||Science, Faculty of > Physics and Physical Oceanography|
|Date:||13 July 2009|
Actions (login required)