Biochemical genetics of orosomucoid

Butler, Ann Marie F. (1986) Biochemical genetics of orosomucoid. Masters thesis, Memorial University of Newfoundland.

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    Available under License - The author retains copyright ownership and moral rights in this thesis. Neither the thesis nor substantial extracts from it may be printed or otherwise reproduced without the author's permission.
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Abstract

This investigation demonstrates that electrophoretically cryptic genetic variation involving methionine residues, can be resolved using site-specific cleavage of the polypeptide chain with cyanogen bromide, followed by SDS-polyacrylamide electrophoresis of the fragments. Orosomucoid, also called α₁-acid glycoprotein, was used as the model to test this approach because the proportion of methionine residues has been reported to be less than unity in this protein, evidence that can be interpreted as due to heterogeneity. A method to purify the model protein, orosomucoid, from as little as 0.5 ml of serum was developed,as a first step in this investigation. Genetic analysis of familial and population material confirmed a polymorphism, Val/Met, at residue 156.

Item Type: Thesis (Masters)
URI: http://research.library.mun.ca/id/eprint/5598
Item ID: 5598
Additional Information: Bibliography: leaves 91-103.
Department(s): Medicine, Faculty of
Date: 1986
Date Type: Submission
Library of Congress Subject Heading: Biochemical genetics; Glycoproteins; Blood protein electrophoresis
Medical Subject Heading: Orosomucoid--genetics; Orosomucoid--isolation & purification; Blood Protein Electrophoresis

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