Barrett, Lisa and Gallant, Maureen E. and Howley, Constance and Bowmer, M. Ian and Hirsch, Geri and Peltekian, Kevork and Grant, Michael D. (2008) Enhanced IL-10 production in response to hepatitis C virus proteins by peripheral blood mononuclear cells from human immunodeficiency virus-monoinfected individuals. BMC Immunology, 9 (13). pp. 1-16. ISSN 1471-2172
PDF (Migrated (PDF/A Conversion) from original format: (application/pdf))
- Published Version
Available under License Creative Commons Attribution.
Background: Multiple immune evasion strategies by which HCV establishes chronic infection have been proposed, including manipulation of cytokine responses. Prior infection with HIV increases the likelihood of chronic HCV infection and accelerates development of HCV-related morbidity. Therefore, we investigated in vitro cytokine responses to HCV structural and non-structural proteins in peripheral blood mononuclear cells (PBMC) from uninfected, HIV-infected, HCVinfected and HIV/HCV-coinfected individuals. Results: Intracellular flow cytometry was used to assess IL-2, IL-10, IL-12, and IFN-γ production by freshly isolated PBMC incubated for 16 hours with recombinant HCV core, non-structural protein 3 (NS3), and NS4 proteins. Anti-HCV cellular responses were assessed in HIV/HCVcoinfected individuals by 3H-thymidine proliferation assay. Exposure to HCV antigens increased IL- 10 production by PBMC, especially in uninfected and HIV-monoinfected individuals. This IL-10 response was attenuated in chronic HCV infection even with HCV/HIV-coinfection. The cells producing IL-10 in response to HCV proteins in vitro matched a PBMC subset recently shown to constitutively produce IL-10 in vivo. This subset was found at similar frequencies in uninfected, HIVinfected, HCV-infected and HIV/HCV-coinfected individuals before exposure to HCV proteins. HCV-specific T cell proliferation was detectable in only one HIV/HCV-coinfected individual who demonstrated no HCV-induced IL-10 response. Conclusion: This pattern suggests that selective induction of IL-10 in uninfected individuals and especially in HIV-monoinfected individuals plays a role in establishing chronic HCV infection and conversely, that attenuation of this response, once chronic infection is established, favours development of hepatic immunopathology.
|Keywords:||Adult; Cell Proliferation; Cytokines; Female; Hepacivirus; Hepatitis C, Chronic; HIV Infections; Humans; Interleukin-10; Leukocytes, Mononuclear; Male; Middle Aged; T-Lymphocytes; Viral Nonstructural Proteins|
|Department(s):||Medicine, Faculty of > Biomedical Sciences|
|Date:||13 June 2008|
Actions (login required)