The analysis of organic constituents in leaves by pyrolysis-gas chromatography and its application to selected environmental effects on plants

Zhang, Hongbing (1993) The analysis of organic constituents in leaves by pyrolysis-gas chromatography and its application to selected environmental effects on plants. Masters thesis, Memorial University of Newfoundland.

[img] [English] PDF - Accepted Version
Available under License - The author retains copyright ownership and moral rights in this thesis. Neither the thesis nor substantial extracts from it may be printed or otherwise reproduced without the author's permission.

Download (7Mb)

Abstract

Forest decline has caused great concern in North America. In recent years, the relationship between environmental stress and the concentration of certain organic compounds in plant leaves, e.g., carbohydrates, phenolics and tannins, has been recognized. Traditional methods for the determination of organic constituents in leaves are very difficult as well as time-consuming. As an alternative method of analysis, Pyrolysis-Gas Chromatography-Mass Spectrometry (PY-GC-MS) can be a rapid and comprehensive method to profile the chemical composition of biomaterials. Furthermore, enhanced structural information of biomolecules containing acidic functional groups has been obtained by a modification of direct pyrolysis - simultaneous pyrolysis methylation (SPM). Initial investigations were focused on the characterization of important classes of biomarkers (e.g., tannins, phenolics) in leaves, and, optimization of sample preparation procedures and pyrolysis conditions. Finally, the pyrolytic techniques were examined as a tool to quantify the compositional changes in leaves of trees which have been subjected to different environment stresses. Results will be reported for three field applications 1) the effect of thinning on balsam fir trees 2) the effect of fertilization on kalmia plant 3) and the effect of ozone on white pine trees. It was found that certain pyrolysates of carbohydrates, tannins and of a dihydrochalcone where good indicators of fertilization and water stress. Two unique pyrolysate bio-markers were discovered in white pine needles as indicators of ozone fumigation levels and ozone damage.

Item Type: Thesis (Masters)
URI: http://research.library.mun.ca/id/eprint/10021
Item ID: 10021
Additional Information: Bibliography: leaves 123-135.
Department(s): Science, Faculty of > Chemistry
Date: 1993
Date Type: Submission
Library of Congress Subject Heading: Botanical chemistry--Technique; Environmental indicators; Foliar diagnosis; Indicators (Biology); Plants--Effect of stress on; Pyrolysis; Tannins.

Actions (login required)

View Item View Item

Downloads

Downloads per month over the past year

View more statistics